Genetic Analysis and Mapping of Novel Cleistogamy Gene,cl7(t) in Rice

【Objective】Identification,genetic analysis and mapping of novel cleistogamy genes in rice would play an important role in understanding the molecular genetic mechanisms of floral development.At the same time,the cleistogamy is an ideal strategy to prevent pollen explosion in transgenic rice.【Method】A rice cleistogamy mutant 8m30 with japonica cultivar H02 background was isolated in M2 population treated with 0.5% EMS(Ethane Methyl Sulfonare).Phenotypic study,genetic analysis and gene mapping by map-based cloning were conducted with the population from F1 of 8m30 and Guanghui102 and its self-cross progenies.【Result】The mutant with lower plant height,compatible plant style and closed paleas at flowering stage.Genetic analysis indicated that the mutant was controlled by a single recessive nuclear gene.The cleistogamy locus was mapped on the long arm of chromosome 7 between the microsatellite markers RM21964 and RM234 with 0.1 cM and 0.3 cM genetic distance,about 160 kb of the physical distance and cosegregated with RM21971,which suggests the mutant locus be novel allele and is tentatively named cl7(t)(Cleistogamy 7(t)).【Conclusion】The 8m30 mutant is controlled by a recessive nuclear gene,which is located on chromosome7 between RM21964 and RM234 with physical distance of 160 kb.