Change of Isoenzymes During Adventitious Bud Differentiation of Watermelon

Peroxidase Isoenzymes and esterase Isoenzymes during adventitious bud differentiation of hua-ling Watermelon were analyzed by using polyacrylamide gel electrophoresis(PAGE) and staining with benzidine reagent.The result showed that while the adventitious bud splits up,peroxidase isoenzymes changed greatly,enzyme content for 20 days reached peak value,and late differentiation appear many bands and heavy content than earlier stage generally.Esterase isoenzymes little changed.The bands was more in later stage than earlier stage.But own content little than the previous stage.