2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF) from Plasmodium falciparum

The putative catalytic domain of an open reading framefrom Plasmodium falciparum with similarity to the ispFgene of Escherichia coli specifying 2C-methyl-d-erythritol2,4-cyclodiphosphate synthase was expressed in a recom-binant E. coli strain. The recombinant protein waspurified to homogeneity and was found to catalyze theformation of 2C-methyl-d-erythritol 2,4-cyclodiphosphatefrom 4-diphosphocytidyl-2C-methyl-d-erythritol 2-phos-phate at a rate of 4.3 mmol·mg