Oligonucleotide linker and applications of oligonucleotide linker in construction of nucleic acid sequencing single-chain cyclic library

The present invention provides an oligonucleotide linker, and particularly relates to uses of the oligonucleotide linker in construction of a single-chain cyclic library in nucleic acid sequencing, and further in sequencing platforms requiring single-chain cyclic libraries. The oligonucleotide linker of the present invention has advantages of high sequencing throughput, high accuracy, and easy operation.