The Overexpression of hDaxx Inhibits Apoptosis Induced by Dexamethasone in HeLa Cells

Objective To study the effects from overexpression of hDaxx on Dexamethasone-induced apoptosis in HeLa cells. Methods An eukaryotic expression vector pEGFP/hDaxx was used to transfect HeLa cells for overexpressing hDaxx. HeLa cells were respectively transfected with pEGFP/hDaxx and pEGFP, and the expression was identified by Western-blot. Furtherly, under fluorescent microscope the expression and localization of GFP-hDaxx fusion protein in HeLa cells was observed. Then, dexamethasone was added to HeLa cells medium for inducing apoptosis, the apoptosis rate of HeLa cells in morphology was counted by Giemsa stain and the difference between the cells transfected by pEGFP and that by pEGFP/hDaxx was analyzed. Results GFP-hDaxx fusion protein was ideally expressed in HeLa cells and localized in nuclei under fluorescent microscope, and GFP-hDaxx fusion protein was identified by Western-blot. Besides, there was a remarkable difference between pEGFP/hDaxx group and dexamethasone control group and pEGFP control group(P0.01), but no remarkable difference between dexamethasone control group and pEGFP control group(P0.05), all of which indicated transient overexpression of hDaxx decreased dexamethasone-induced apoptosis rate of HeLa cells. Conclusion Daxx is a regulatory protein in nuclei, GFP-hDaxx fusion protein is expressed in HeLa cells and localized in nuclei, and transient overexpression of hDaxx inhibits dexamethasone-induced apoptosis in HeLa cells.