Bioactive Steroids from the Whole Herb of Euphorbia chamaesyce

Three new ergostane-type steroids, 3β-hydroxy-4α,14α-dimethyl-5α-ergosta-8,24(28)-dien-11-one (1); 3β,11α-dihydroxy-4α,14α-dimethyl-5α-ergosta-8,24(28)-dien-7-one (2); and 3β,7α-dihydroxy-4α,14α-dimethyl-5α-ergosta-8,24(28)-dien-11-one (3), were isolated, together with two known triterpenoids, wrightial and lup-20(30)-ene-3β,29-diol from the whole herb of Euphorbia chamaesyce. Compound 3 showed a potent inhibitory effect on Epstein−Barr virus early antigen activation induced by the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA).