Characterization and quantification of racemic and meso-ethylenediamine-N,N'-bis(2-hydroxy-5-sulfophenylacetic) acid/iron (III) by ion-pair ultra-high performance liquid chromatography coupled with diode array detector and electrospray tandem mass spectrometry.

Abstract EDDHSA/Fe is a promising substitute of EDDHA/Fe to fight iron chlorosis. o , o -EDDHSA structure contains two chiral carbons giving the racemic and meso couples of stereoisomers. Ion-pair HPLC and UHPLC–UV/Vis–ESI-MS/MS methods were developed for the determination of racemic and meso - o , o -EDDHSA/Fe in commercial samples of chelates. The lack of a commercial EDDHSA standard was overcome by sulfonation of a commercial available o , o -EDDHA standard and subsequent quantification by 1 H-NMR. Assignment of configurations was carried out starting from racemic and meso - o , o -EDDHA/Fe by direct sulfonation to give the corresponding o , o -EDDHSA/Fe isomers. The performances of these methods were assessed in terms of intra and inter-day precision, linearity and selectivity. The high selectivity and lower detection limit (nanomolar) of the UHPLC–ESI-MS/MS method could allow to deepen the knowledge relative to meso and rac - o , o -EDDHSA/Fe interactions with plants, its fate in different soil conditions, its mobility and other environmental aspects.