TOXICITY RESEARCH OF SPINE POISONING FISH FREQUENTLY FOUND IN CHINA COASTAL WATERS ──EFFECTS OF INJECTION WITH CRUDE VENOM EXTRACTION OF STONEFISH (INIMICUS JAPONICUS) ON THE STRUCTURE AND METABOLISM IN THE RAT WOUND TISSUES

in order to study the mechanism of venomous effects of Inimicus japonicus, in this paper, we used Histologic, Histochemical techniques and Immunoradioassay (RIA) to study the venomous effects on the structure and the metabolisms of Acetylcholinesterase (AchE) and Succinate Dehydrogenase (SDH), and on the contents of Substance P (SP) and Somatostatin (SS) in the wound tissues. The results show that the injection of the crude venom from Inimicus japonicus could cause the wound tissues severe swelling, damage and hemorrhage, decreased the SDH activities, specially in the red and intermediate muscle cells, but markedly increased the AchE activities and the contents of SP. This indicats that the cause of severe pain and swelling stung by Inimicus japonicus may be related to SP and the cause of paresthesia may be mediated by accelerating the degradation of Ach,muscular and nervous fiber damage induced by crude venom.