Effects of lentivirus-mediated stromal interaction molecular 1 gene knock-down on apoptosis of SMMC7721 cells

Objective To observe the effect of stromal interaction molecular 1 ( Stim1 ) down-regulation on apoptosis of hepatocellular carcinoma (HCC) cells.Methods The siRNA to Stiml was designed and synthesized,inserted to lentiviral vector.The third generation envelope system was used to produce lentiviral particles.Lentivirus infection of SMMC7721 was performed at MOI =2.The expression of Stiml was detected by using quantitative polymerase chain reaction (PCR) and Western blotting.Annexin V and proliferation index ( PI ) staining was used to evaluate apoptosis of SMMC7721 cells.Results Sequencing confirmed Stiml siRNA fragment was linked into interference vector.The expression of Stiml in infected cells was knocked down by above 75％ detected by quantitative PCR and Western blotting.FACS showed that SMMC7721 apoptosis rate was increased with knockdown of Stiml.Conclusion Down-regulation of Stiml promotes apoptosis of SMMC7721 cells. Key words: Carcinoma, hepatoeellular; Stromal interaction molecular 1; RNA interference; Apoptosis