Optimization of Supercritical CO2 Fluid Extraction of Flavonoids from Spina Gleditsiae by Response Surface Method

In order to optimize the supercritical CO2 fluid extraction of flavonoids from spina gleditsiae, single-factor experiments were utilized to investigate the influences of the extraction temperature, extraction pressure, and entrainment dosage firstly. Then the optimal extraction process for flavonoids extraction was determined by response surface methodology and the quadratic mathematical model was established which reliability was finally verified. The optimal supercritical CO2 fluid extraction conditions were as follows: entrainment dosage of 36mL, extraction temperature of 48°Cand extraction pressure of 40Mpa. Under this condition, the yield of flavonoids from spina gleditsiae was 0.793%.