Rapid chromatographic quantitation of glycosylated haemoglobins.

Abstract We have developed a rapid chromatographic method for determination of glycosylated haemoglobins by high-performance liquid chromatography with a new cation-exhange column. The haemoglobins are eluted with a three-step gradient in 7 min, and total assay time including re-equilibration of the column is 15 min. The method permits separation and quantitation of HbAlc, even in the presence of elevated levels of HbF. HbAla, Alb and A2 can also be determined. The results correlate well ( r = 0.94) with those obtained by the macro-column method of Trivelli et al. [ New Engl. J. Med. , 284 (1971) 353] for determination of HbAlc. The method has been fully automated by the use of an automatic injector. The within-assay and between-assay coefficient of variation of the method is 2–3%.