Determination of Monoester-alkaloids from Aconiti Kusnezoffii Radix Cocta in Xiaojin Wan

Objective: To compare monoester-alkaloids content of different batches of Xiaojin Wan from different manufacturers and provide a reference for clinical drug safety of this preparation. Method: Determination of monoester-alkaloids from Aconiti Kusnezoffii Radix Cocta in Xiaojin Wan was established, including benzoylmesaconine,benzoylaconine and benzoylhypacoitine; mobile phase was consisted of acetonitrile-20 mmol·L- 1sodium dihydrogen phosphate( adjusted pH to 6. 5 with triethylamine)( 47∶ 53),detection wavelength was set at235 nm. The content of these three components in 8 batches of Xiaojin Wan from four manufacturers was compared. Result: Linear ranges of these three components were 4-60 mg ·L- 1,recoveries were 98.42%,101. 79% and 102. 13%,respectively. Total content of these three components was up to 214. 718 μg·g- 1with the lowest value of 18. 452 μg·g- 1. Conclusion: There is a big difference among 3 kinds of monoester-alkaloids from different manufacturers and different batches of the same manufacturer,which may be related to quality of raw materials,preparation process,production equipment and other factors.