Volumetric spiral chemical shift imaging of hyperpolarized [2-13c]pyruvate in a rat c6 glioma model
2016
Purpose
MRS of hyperpolarized [2-13C]pyruvate can be used to assess multiple metabolic pathways within mitochondria as the 13C label is not lost with the conversion of pyruvate to acetyl-CoA. This study presents the first MR spectroscopic imaging of hyperpolarized [2-13C]pyruvate in glioma-bearing brain.
Methods
Spiral chemical shift imaging with spectrally undersampling scheme (1042 Hz) and a hard-pulse excitation was exploited to simultaneously image [2-13C]pyruvate, [2-13C]lactate, and [5-13C]glutamate, the metabolites known to be produced in brain after an injection of hyperpolarized [2-13C]pyruvate, without chemical shift displacement artifacts. A separate undersampling scheme (890 Hz) was also used to image [1-13C]acetyl-carnitine. Healthy and C6 glioma-implanted rat brains were imaged at baseline and after dichloroacetate administration, a drug that modulates pyruvate dehydrogenase kinase activity.
Results
The baseline metabolite maps showed higher lactate and lower glutamate in tumor as compared to normal-appearing brain. Dichloroacetate led to an increase in glutamate in both tumor and normal-appearing brain. Dichloroacetate-induced %-decrease of lactate/glutamate was comparable to the lactate/bicarbonate decrease from hyperpolarized [1-13C]pyruvate studies. Acetyl-carnitine was observed in the muscle/fat tissue surrounding the brain.
Conclusion
Robust volumetric imaging with hyperpolarized [2-13C]pyruvate and downstream products was performed in glioma-bearing rat brains, demonstrating changes in mitochondrial metabolism with dichloroacetate. Magn Reson Med, 2015. © 2015 Wiley Periodicals, Inc.
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