Effect of zinc-bearing palygorskite on rumen bacterial diversity in vitro

Objective The aim of this experiment was to study the effects of zinc-bearing palygorskite (Zn-Pal) on rumen bacterial diversity in vitro . Methods We prepared Zn-Pal by the ion-exchange, and evaluated the compositions of bacterial communities in 60 samples based on 16S rDNA genes. Results We obtained a total of 1490959 effective sequences and 87662 OTUs. The bacterial diversity in the treatment Ⅳ group increased at 24 h, and the abundance of the treatment Ⅳ group increased at 48 h. Bacterial community composition analysis shows that the dominant phyla were Bacteroidetes, Firmicutes, Proteobacteria and Lentisphaerae. Compared with the control group, Firmicutes in treatment groups significantly increased ( P P Prevotella had no significant difference between the control and treatments. The relative abundance of Treponema in the treatment Ⅳ group was significantly higher at 48 h than in the control group ( P Victivallis and Pseudobutyrivibrio in treatment Ⅳ was lower. Conclusion Zn-Pal may affect rumen fluid bacterial diversity in dairy cows, and the degrees of this influence varied with the dose and time of Zn-Pal.