Studies on Adventitious Bud Proliferation of Chinese Fir

Taking mature shoot sections of superior clones as test materials,an in vitro procedure for adventitious buds’induction in Chinese fir(Cunninghamia lanceolata) was set up.In this research,some factors,such as the type of plant growth regulator,genotype of the explants and activated charcoal in the medium,especially the methods of buds’inoculation,were studied for adventitious buds and proliferation.The results showed that when the concentration of 6-BA came to 1.5 mg·L-1,it could effectively improve the proliferation of adventitious buds.However,while the concentration of 6-BA was too high,it would inhibit the buds’proliferation and they were easily vitrified.Removal of apical dominance could significantly increase the efficiency of buds’induction.And the efficiency differed a lot according to different genotypes.Using BA,KT,and NAA in combination was better for elongation of buds than using individual plant growth regulator;and proper using of activated charcoal could improve buds’elongation.