Detecting Citrullinemia in Holstein calves using PCR-SSCP analysis.

【Objective】 The study established a method to detect Citrullinemia by polymerase chain reaction-single strand conformation polymorphism analysis.【Method】 A pair of primers were designed and synthesized according to nucleotide sequences of the exon 5 for argininosuccinate synthesize gene in Holstein Calves,published in Genbank and under accession number:M2619.Polymerase chain reaction-single strand conformation polymorphism was used to analyze exon 5 for argininosuccinate synthesize of 173 Holstein Calves in Beijing,and the results were verified by polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing.【Result】 One of the 173 Holstein Calves was Citrullinemia carrier,and the carrying rate was 0.58%.This result was consistent with that of the polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing.【Conclusion】 A method detecting Citrullinemia by polymerase chain reaction-single strand conformation polymorphism analysis was established.This method is not only simple and convenient,but also has a high accuracy and low cost,which is more suitable for large-scale Citrullinemia investigation.