[Inhibition effect of bone marrow mesenchymal stem cells on T-lymphocyte proliferation through up-regulation of CD8+CD28- T cells].

The aim of this study was to explore effect of CD8+CD28-T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+T cells were sorted by magnetic beads; the T-lymphoc yte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28-T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhib itory eff ect depended on the amount of MSC; the data of FACS indicated that in the CD8 +T cells co-cultured with MSC, CD8+CD28-T cells were up-regulated signific antly, com pared with the non-treated CD8+T cells. In conclusion, MSC perform their imm unosuppressive function by up-regulation of CD8+CD28-T cells.