An approach for enhancing the production of human-like collagen II by enlarging the metabolic flux at pyruvate node

As a key metabolite of glycolysis, Pyruvate affects cell growth and metabolism directly. In order to enhance the production of human-like collagen (HLC) II, pyruvate was added into fermentation broth, and response surface methodology (RSM) to optimize the addition time and dosage of pyruvate and the concentration of phosphate buffer. And the results of PB-design showed that only two variables- the dosage of pyruvate and the concentration of buffer-had significant effects on the production of human-like collagen. Quadratic polynomial models were established after analyzing the results of full factorial central composite design. The optimal dosage of pyruvate and the concentration of buffer were 8.23 mmol·L -1 and 0.17 mol·L -1 respectively. Under the optimal cultivation condition, Cell OD600 was increased 28.72%; the maximal production of human-like collagen could be up to 0.29 g·L -1 which was 11.54% higher than that of the control group 0.26 g·L -1 (the highest production of the former study).