Identification of a Tyrosine-based Motif (YGSI) in the Amino Terminus of Nramp1 (Slc11a1) That Is Important

-acting sequences responsiblefortargetingtolysosomes.Forthis,weconstructedandstudiedNramp1/Nramp2 chimeric proteins where homologousdomainsofeachproteinwereexchanged.Chimerasexchangingthe amino- (upstream TM1) and carboxyl-terminal (down-stream TM12) cytoplasmic segments of both transporters werestablyexpressedinporcineLLC-PK