Variation of Microsatellite Markers in a Collection of Lao's Black Glutinous Rice (Oryza sativa L)

The genetic diversity of 74 genotypes, including black glutinous rice (BGR) from Lao’s germplasm was assessed using 24 microsatellite markers. A total of 75 alleles were detected at the 24 microsatellite markers. The number of alleles per marker varied from 2 to 7 with an average of 3.1 alleles per locus. The gene diversity (GD) and polymorphism information content (PIC) ranged from 0.18 to 0.79 and 0.17-0.76 respectively, and the allele frequency (AF) ranged from 0.36 to 0.90. The markers were able to classify rice genotypes into four groups; indica rices were put in the three groups while the other group consisted of tropical japonica rice. The first indica group (G1) included 24 genotypes of BGR and five genotypes of white rice. Most of genotypes in this group have thick culms, broad leaf blades, large and bold grain shapes, and some of them have purple coloration on all vegetative parts. In other varieties with black pericarp, all other plant parts are green. Indica group 3 (G3) included 25 genotypes of BGR. The special characters of this subgroup were small and slender culms, narrow short leaves, purple leaf margins, purple leaf tips, and purple stripes on leaf blades and sheathes. However, the markers used could not differentiate between LG 8215 and LG 7937. Indica group four (G4) consisted of eight genotypes of white rice and the four check varieties. Group (G2) consisted of five white rices, four BGRs, and three check varieties.