Device and method for the propagation and characterization of cells for subsequent

A device for the propagation of cells, and for subsequent characterization of the cells, comprising: - a planar substrate on which at least one reaction site are provided, each of the at least one reaction site comprising a central hydrophilic region, which is externally surrounded by a first hydrophobic region, which in turn is externally surrounded by a central hydrophilic region of the outside from a second hydrophobic region is surrounded and - passage channel has an attachment which is detachably connected to the substrate and which having at least one an upper opening and a lower opening, when the attachment is connected to the substrate, at least one of the side facing the substrate lower opening (s) of at least a through channel of the cap is disposed above a central hydrophilic portion of a reaction site such that in the at least one passageway located fluid may at least wet a portion of the central hydrophilic region of the reaction site, the liquid, however, can not wet the surrounding average hydrophilic area of ​​the reaction site.