In ovulo embryo culture of stenospermocarpic grapes

Ovules of 14 seedless cultivars, collected 41-49 d after anthesis, were excised and in vitro cultured on 2 different media: NN + 1 µM GA3 + 10 µM IAA and NN + 1 µM GA3 + 20 µM IAA + 2 g/l activated charcoal. Plant development occurred at variable rates; the best percentages were found in Flame Seedless, Perlon, Imperatrice, Carina, Perlette and Ruby Seedless. Genotype resulted to be the main factor affecting ovule response to culture, but also the medium and the interaction genotype x medium influenced it. The addition of 2 g/l activated charcoal to the medium, tested on the cv. Perlette, and the type of added auxin (IBA, IAA, NAA), tested on the cvs Perlette and Sultanina, enhanced plantlet development.