Effectiveness of Grape (Vitis Vinifera L.) Seed Extracts on Fungi and Bacteria Management
2018
Grape ( Vitis vinifera L.) seeds from 3 grape varieties were powdered and the fatty material was extracted. These extracts were tested for grape storage restriction fungi Botrytis cinerea, Alternaria alternata , Aspregillus niger and Penicillium expansum and antibacteria activity for Gram-negative Escherichia coli , Pseudomonas aeruginosa , Klebsiella pneumonia , and Gram-positive Enterococcus faecalis , Streptococcus pneumonia and Staphylococcus aureus by pour plate method. The grape seed extracts (GSE) were tested against peri-implantitis microflora. Suspension of microorganisms was made in sterile normal saline and adjusted to 0.5 Macfarland standard (10 8 Cfu mL -1 ). From the stock of 65536 mg mL -1 GSEs, serial dilutions were made up to 4 mg mL -1 . It was found that, no effective restriction and/or inhibition for tested fungi and Gram-negative Escherichia coli 35218, Klebsiella pneumonia 700603, Pseudomonas aeruginosa 27853, and Gram-positive Enterococcus feacalis 51299 bacteria while Gram-positive Staphylococus aureu 44300 was inhibited at 32768 μg mL -1 GSE of ‘Muskule’ variety and GSE 65536 μg mL -1 GSE of ‘Okuzgozu’ and Streptococcus pneumonia 49619 bacteria were inhibited at 2048 μg mL -1 4096 μg mL -1 and 32768 μg mL -1 concentration of ‘Kara Dimrit’, ‘Okuzgozu’ and ‘Muskule’ GSEs. The results of the study showed that GSEs has potential antimicrobial effects which can be further studied.
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