Two-photon phosphorescence lifetime microscopy of intestinal capillary pO2 and RBC flux

2021 
Quantifying intestinal microvascular oxygenation may shed light on origin and progression of intestinal diseases as well as the role of the gut microbiota. In this work, we developed a method for depth-resolved microscopic measurements of partial pressure of oxygen (pO2) and Red Blood Cell (RBC) flux in the capillaries of intestinal wall in mouse cecum. Capillary pO2 and RBC flux were measured in healthy mice as a function of depth during normoxia and hyperoxia. Our results show low mean capillary RBC flux and relatively uniform capillary pO2 across the cecum wall depth.
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