Growth activity of osteoblast on a novel strontium incorporated calcium sulfate

Objective:To investigate the growth activity of osteoblast on a novel strontium incorporated calcium sulfate and make comparison with normal calcium sulfate material. Methods:Osteoblast was inoculated on samples and cell proliferation was measured on the 1st,3rd,5th days,and the activities of ALP and osteocalcin were observed on the 5th day. And microcosmic morphology of osteoblast was observed by scanning electron microscopy(SEM). Results:Osteoblast grows robustly on tested material. Cell quantity on the surface of novel material was obviously higher than normal calcium sulfate material(P 0.05). The activity of ALP and osteocalcin on novel material was 57.8% and 40.2% higher than on normal calcium sulfate material respectively(P0.05). On strontium incorporated surface,osteoblast spread well. Cells were polygonal with abundant cytoplasm and the morphology was active. Conclusion:Strontium incorporated calcium sulfate can sustain robust growth activity of osteoblast,which is promising to be used for bone substitute materials.