Phyllobacterium zundukense sp. nov., a novel species of rhizobia isolated from root nodules of the legume species Oxytropis triphylla (Pall.) Pers.
2018
Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating from Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that the novel isolates were phylogenetically closest to the type strains
Phyllobacterium sophorae
LMG 27899T,
Phyllobacterium brassicacearum
LMG 22836T,
Phyllobacterium endophyticum
LMG 26470T and
Phyllobacterium bourgognense
LMG 22837
T while similarity levels between the isolates and the most closely related strain
P. endophyticum
LMG 26470T were 98.8–99.5 %. The recA and glnII genes of the isolates showed highest sequence similarities with
P. sophorae
LMG 27899T (95.4 and 89.5 %, respectively) and
P. brassicacearum
LMG 22836T (91.4 and 85.1 %, respectively). Comparative analysis of phenotypic properties between the novel isolates and the closest reference strains
P. sophorae
LMG 27899T,
P. brassicacearum
LMG 22836T and
P. endophyticum
LMG 26470T was performed using a microassay system. Average nucleotide identities between the whole genome sequences of the isolates Tri-38 and Tri-48T and
P. sophorae
LMG 27899T,
P. brassicacearum
LMG 22836T and
P. endophyticum
LMG 26470T ranged from 79.23 % for
P. endophyticum
LMG 26470T to 85.74 % for
P. sophorae
LMG 27899T. The common nodABC genes required for legume nodulation were absent from strains Tri-38 and Tri-48T, although some other symbiotic nod and fix genes were detected. On the basis of genotypic and phenotypic analysis, a novel species, Phyllobacterium zundukense sp. nov. (type strain Tri-48T=LMG 30371T=RCAM 03910T), is proposed.
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