Penapisan Mikroba Laut Perombak Senyawa Nitril dan Protein yang Diisolasi Dari Spons di Perairan Ternate

ABSTRACT Screening of Nitrile and Protein-Degrading Marine Bacteria Isolated from Sponge in Ternate Sea Water. Thirty three marine bacteria have been isolated from marine sponge in Ternate by enrichment culture. Screening bacteria-degrading nitrile was done by microtitter plate method based on growth ability tested by Iodonitrotetazolium chloride. Product of nitrile degradation was determined by Gas Chromatography (GC) and the potential bacteria-degrading protein was also screened by using selected media which contained casein. The results showed that twenty one isolates were able to show the clearing zone in selected media. Five isolates capable of utilizing acetamide as the sole source of carbon and nitrogen. Acetate and ammonia produced for hydrolysis acetonitrile by using resting cell of Lysobacter sp. Key words: Nitrile, bacterium, sponge, Ternate