Изучение длины теломер и колониеобразующей способности гемопоэтических стволовых клеток пуповинной крови при криоконсервировании

The study was focused on proliferative capacities of human hematopoietic cord blood stem cells at two different methods of cryopreservation. The proliferative potential was determined by measurement of telomere length and colonyforming cell assay. The telomere length was estimated by technique that combined flow cytometry and fluorescence in situ hybridization on Beckman Coulter Cytomics FS-500. We used the Telomere PNA Kit/FITC for Flow Cytometry for measuring telomeric sequences as well as MethoCult® GF H4435 in colony-forming cell assays. The programmed Planer Cryo freezer and direct transfer into liquid nitrogen vapour were used for cryopreservation of samples. The correlation between telomere length and capacity of cells to forming colony after thawing were established. The programmed freezing showed advantage over freezing by direct transfer cells into liquid nitrogen vapour by better keeping of proliferative capacity hematopoietic cells.