Synthetic phospholipids as specific substrates for plasma endothelial lipase

Abstract We designed and prepared synthetic phospholipids that generate lyso-phosphatidylcholine products with a unique mass for convenient detection by LC–MS in complex biological matrices. We demonstrated that compound 4 , formulated either as a Triton X-100 emulsion or incorporated in synthetic HDL particles can serve as a substrate for plasma EL with useful specificity.