Effect of strain and nutritional density of the diet on the water-protein ratio, fat and collagen levels in the breast and legs of broilers slaughtered at different ages

Abstract Many requirements are necessary to meet the European Union rules to export poultry, including the amount of physiological water and water-protein ratio (WPR) in carcasses. Therefore, the aim of this study was to identify if strain, nutrition, and age affect the amount of collagen and fat and the WPR in cuts and verify whether the latter meets the international export standards. A total of 3,240 male chicks were housed in a completely randomized design in a 3 × 3 × 5 factorial arrangement, which included 3 nutritional densities (regular, medium, and high), 3 strains (021 Embrapa and 2 commercial strains identified as A and B), and 5 ages. Twelve broilers from each treatment (totaling 540 birds) were slaughtered at 28, 35, 42, 49, and 56 D of age to determine collagen and fat levels and WPR (through the calculation of moisture and protein percentage) in broiler breasts and legs using the near-infrared spectroscopy method. The use of feeds with different nutritional densities presented in this study has no effect on the WPR in the breast and legs of broilers slaughtered between 28 and 56 D of age. However, nutritional density influences liveweight and percentage of fat in the breast and legs. Collagen percentage in the legs decreases with increasing nutritional density. The 021 Embrapa strain cuts present a lower WPR than those of other commercial strains. However, the values found for all strains studied are within the limits of the Europe Union and Brazilian legislations. The liveweight, breast weight, leg weight, and leg fat increases linearly with age. Quite the opposite, water protein ratio, breast fat level, and breast collagen level decrease linearly with age. Leg WPR and leg collagen level are not affected by age. Despite the differences found for strains, nutritional densities and age readers should be aware that these factors may interact with each other depending on the response variable studied.