Influence of different local anesthetics on the in vitro collagen synthesis of periodontal fibroblasts

: The influence of different local anesthetic fluids in an in-vitro-experiment of periodontal ligament cells was researched. Mepivacain, articain, lidocain and prilocain were used in the study. The common commercial local anesthetic fluids were in a proportion 1:1, 1:10 and 1:30 diluted, before they were placed in the incubator with cultures of periodontal ligament cells. At the 1st and 14th day after the experiment the synthesis of collagen type I, III, V and VI were determined with an ELISA. The mepivacain dilution of 1:30 had, compared with the controls the slightest influence on the collagen synthesis of periodontal ligament cells. Further clinical investigations must show if through dilution of the local anesthetic solution an adequate anesthesia can be reached.