Anti-proliferation effect of guanosine on HCT 116 cells involves MAPK and AMPK pathways

ABSTRACTThis study aims to investigate the mechanisms associated with the anti-proliferation effect of guanosine on human colon carcinoma HCT 116 cells. In this study, guanosine induced more drastic cell cycle arrest effect than cell death effect on HCT 116 cells. The cell cycle arrest effect of guanosine on HCT 116 cells appeared to be associated with the increased activation of mitogen-activated protein kinases (MAPK) such as ERK1/2, p38 and JNK. The decreased of AMP-activated protein kinase (AMPK) activation and cyclin D1 expression was also involved. Thus, the anti-proliferation of colon cancer cells of guanosine could be mediated by the disruption of MAPK and AMPK pathways.