METHOD FOR CORD BLOOD MONONUCLEAR CELLS (cbMNC) EXPANSION ex vivo IN PRESENCE OF MULTIPOTENT MESENCHYMAL STROMAL CELLS (MMSCS)

FIELD: medicine. SUBSTANCE: what is presented is a method for cord blood mononuclear cells (cbMNC) expansion ex vivo in the presence of multipotent mesenchymal stromal cells (MMSCs) involving culturing MMSCs from the stromal vascular fraction of fatty tissue to reach a monolayer at the concentration of O 2 in the medium of 5%, adding a cbMNC suspension to the MMSCs monolayer, culturing for 72 hours at the concentration of O 2 in the medium of 5%, selecting the unattached cbMNCs and replacing the medium, continuing the MMSCs culturing with the attached cbMNCs for 7 days at the concentration of O 2 in the medium of 5%. EFFECT: invention can be used in preparing the stem cell cultures for needs of regeneration medicine. 3 dwg, 3 tbl, 1 ex