[Apoptosis-inducing Effect of 8-Bromo-7-Methoxychrysin on K562 cells].

This study was purposed to investigate the apoptosis-inducing effect of 8-bromo-7-methoxyhysin(BrMChR) on leukemia K562 cells as well as the variation of caspase-3 activity and phosphorylated Akt(p-Akt) expression of K562 cells during the process of apoptosis.MTT assay was used to determine the inhibitory effect of BrMChR on proliferation of K562 cells.Cell apoptosis was assayed by AO/EB staining under fluorescent microscope and flow cytometry with Annexin V-FITC/PI staining.The expression level of p-Akt was measured by Western blot.The results showed that BrMChR had the inhibitory effect on proliferation of K562 cells and could induce apoptosis of these cells in dose-dependent manner,and these effects were significantly stronger than ChR.After treatment of K562 cells with 3 μmol/L ChR for 12 hours,the apoptosis rate was only 3.68%,but the apoptosis rate of K562 cells treated with 3 μmol/L BrMChR was 21.8%.In the same time,the caspase-3 activity significantly increased(p0.05),but the expression of p-Akt was down-regulated(p0.01).It is concluded that BrMChR can induce apoptosis of K562 cells and with effect stronger than chR.P-Akt may participate in the apoptosis process of K562 cells induced by BrMChR.