DNA-dependent ATPase B of FM3A cells: Its separation from DNA polymerase α

Abstract One form of DNA-dependent ATPase (DNA-dependent ATPase B) has been purified from FM3A cells. In this report, we describe the association of DNA polymerase α activity with DNA-dependent ATPase B through a series of purification steps and the final separation of the two enzymes by glycerol gradient centrifugation operated at a low salt concentration.