Nano-Bio zinc synthesized by Bacillus subtilis modulates broiler performance, intestinal morphology and expression of tight junction's proteins

Abstract Nano-zinc particles was synthesized using the Bacillus subtilis and the particles were characterized by transmission electron microscopy (TEM) methods. Treatment was applied in a completely randomized design with three sources of zinc (zinc sulfate, organic zinc, and nano-bio zinc) along with a control diet nonsupplemented with zinc, resulting in a total of 4 treatments with 6 replicates of 10 chicks. Performance criteria, ileum morphometry and microbial population and jejunum tight junction proteins relative expression were evaluated during the trial. The particle size of the synthesized zinc nanoparticles ranges 80 to 350 nm, with crystalline spherical or roughly spherical forms. Inclusion of nano-bio zinc increased BW and improved FCR compared to the organic and ZnSO4. Zinc supplementation meaningfully (P