Lentivirus-mediated transfection of chondroitinase ABC gene without the bacterial leader sequence enables long-term secretion of functional chondroitinase ABC in human bone marrow stromal cells

Yang Guo,Michael Klüppel,Hao Tang,Sheng Tan,Peidong Zhang,Zhenzhou Chen

Lentivirus-mediated transfection of chondroitinase ABC gene without the bacterial leader sequence enables long-term secretion of functional chondroitinase ABC in human bone marrow stromal cells

2016

Yang Guo
Michael Klüppel
Hao Tang
Sheng Tan
Peidong Zhang
Zhenzhou Chen

Objective To test the feasibility of secretion of functional chondroitinase ABC (ChABC), a bacterial enzyme that promotes axonal regeneration after spinal cord injury, from human bone marrow stromal cells (hBMSCs).

Keywords:

Bone marrow
Gene expression
Biology
Molecular biology
Chondroitin ABC lyase
Secretion
Stromal cell
Transfection
Mesenchymal stem cell
Real-time polymerase chain reaction
Lentivirus

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