Effects of DLC-1 gene transfection on HT29 cell invasion:the role of ROCK signal pathway

Objective To investigate the effects of frequently deleted in liver (DLC-1) gene transfection on HT29 cell invasion.Methods Recombinant pcDNA3.1 vector containing the DLC-1 gene was constructed and transfected into HT29 cell line.HT29 cell was treated with Rho-associated kinase(ROCK) inhibitor Y27632.The expression of p-MLC was examined by Western blotting.The invasion ability was detected by transwell.Results The HT-29 cells rarely expressed DLC-1 mRNA and the transfected HT-29 cells could express DLC-1 mRNA steadily.Significant down-regulation of p-MLC expression and decreased invasive ability of DLC-1 were seen in groups of transfected and ROCK inhibited HT29 cells.Conclusion Overexpression of DLC-1 gene caused significantly down-regulation of ROCK activity,which may further inhibit the invasiof HT29 cells.