Cu(II) complexation does not affect oxytocin action on pregnant human myometrium in vitro

Abstract Objective Copper may influence the in vivo and in vitro uterine activity. Recent evidence shows that cupric ions can easily form complexes with oligopeptides like oxytocin (OXT). The high complex stability in vitro suggests a possibility of complex formation in vivo . Study design In vitro isometric contractions were recorded in uterine tissues from pregnant women undergoing caesarean sections and the effect of OXT and the Cu–OXT complex on isolated human pregnant myometrium was investigated. Results In the concentration range from 10 −14 to 10 −6  M of OXT alone, pre-formed Cu–OXT complex, and OXT following sample preincubation with Cu(II) salt, nosignificant differences were observed for the following parameters of pregnant uterine smooth muscle contraction: the area under the curve, frequency and amplitude of contraction. Conclusion The binding of Cu 2+ ions does not abolish the ability of OXT to interact with oxytocin receptors and stimulate myometrial contraction in vitro.