Determination of oxymatrine in Compound Kushen Colon-specific Capsula by capillary electrophoresis.

Objective To establish an HPCE method for the determination of oxymatrine in Compound Kushen Colon-specific Capsula.Methods Amphetamine sulfate was used as the internal standard.The separation was obtained with silica capillary column(50 cm × 50 μm,effective length 45 cm),30 mmol/L phosphate buffer(pH 5.8)at a constant voltage of 20 kV,and temperature at(25±1)℃,the detective wavelength 214 nm.Results The linear determination range was 30—240 μg/mL and the average recovery and RSD were 99.54% and 1.85%,respectively.Conclusion The method is simple and accurate,and can be used for quality control of Compoud Kusheng Colon-specific Capsula.