Extraction of ultra-low gossypol protein from cottonseed: Characterization based on antioxidant activity, structural morphology and functional group analysis

Abstract Cottonseed can be considered as a potential candidate to act as an important protein source. However, toxic compound "gossypol" is the main hurdle in utilization of this potential source of protein. The current study aims to optimize the extraction of cottonseed protein isolate (CSPI) from defatted cottonseed meal (CSM) using Box-Behnken design (BBD) with ultra-low gossypol concentration (ULGC,