The regulatory effects of L. plantarum peptidoglycan microspheres on innate and humoral immunity in mouse

AbstractObjective: To study the effects of Lactobacillus Plantarum cell wall peptidoglycan (LPG) microspheres on mouse intestinal flora changes, peptidoglycan recognitions protein (PGRP) and cytokines expression levels. Method: Plate counting was used for enumeration of the intestinal flora. Real-time PCR was used for quantification PGRP in different tissues. Cytokines content were determined by ELISA kits. Result: The mouse administered orally with LPG microspheres showed significantly higher number of Lactobacillus and Bifidobacterium in cecum contents (P < 0.01). The amount of PGRP expression in different organs was highest in LPG microspheres-treated group. IL-4, 12, IFN-γ, TNF-α contents in serum from LPG microspheres-treated mouse were significantly higher than those in normal saline-treated group (P < 0.01). Conclusion: This study shows that the LPG microspheres can regulate intestinal flora imbalance and improve systemic immunity, improve both Th1 and Th2 immune response, which provide some basis ...