Adaptive Optics Light-Sheet Microscopy For Functional Neuroimaging

Antoine Hubert,Fabrice Harms,Sophia Imperato,Vincent Loriette,Cynthia Veilly,Xavier Levecq,Georges Farkouh,François Rouyer,Alexandra Fragola

Adaptive Optics Light-Sheet Microscopy For Functional Neuroimaging

2021

Antoine Hubert
Fabrice Harms
Sophia Imperato
Vincent Loriette
Cynthia Veilly
Xavier Levecq
Georges Farkouh
François Rouyer
Alexandra Fragola

We present a novel implementation of extended-scene adaptive optics for light-sheet microscopy based on direct wavefront sensing for fast aberration correction. We report AO-enhanced functional images of GCaMP neurons in the live drosophila brain.

Keywords:

Microscopy
Wavefront
Adaptive optics
Light sheet fluorescence microscopy
GCaMP
Functional neuroimaging
Optics
Functional imaging
Neuroimaging
Physics

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