Determination of Content of Coenzyme Q10 in Copolymer Micellers with SDS Solubilization

The total content of nutrient is an important index for evaluating the quality of casein-dextran copolymer micellers as a delivery system.Sodium dodecyl sulfate(SDS) as a surfactant can be used to disorganize copolymer micellers and make coenzyme Q10 solubilize in water for quantitative analysis.In this study,the recovery rate of coenzyme Q10 from blank casein-dextran copolymer micellers ranged from(98.77±1.60) % to(95.90±0.80) % with conditions as follows: SDS concentration 0.31 mol/L,incubation temperature 30 ℃,sodium borohydride(7 mg/mL) dL,reduction time 3~5 min.The analysis of synchronous and exogenous fluorescence spectra indicated that the hydrophobic region of casein unit of copolymer was partly changed during solubilization at SDS concentration form 0 to 0.5 mol/L.The interaction between SDS and hydrophobic region was not influenced by temperature when the concentration of SDS was 0.31 mol/L.