Application of TLC densitometric method for simultaneous estimation of binary mixture of phenazopyridine hydrochloride and trimethoprim in their tablet dosage form

In the present work an accurate and precise TLC densitometric method has been developed for simultaneous determination of PHZ and TMP in their bulk and dosage form The proposed method based on determination of the UV visualized bands after TLC separation of PHZ and TMP The studied drugs were quantitatively separated on F silica gel plates using mobile phase consists of methanol toluene ethyl acetate by volume with UV detection at nm over a concentration range of ndash and mu g spot with mean percentage recoveries plusmn and plusmn for PHZ and TMP respectively The proposed method has been validated according to ICH guidelines The validity of the proposed method was further evaluated by applying standard addition technique The obtained results were statistically compared with the reported method showing no significant difference with respect to accuracy and precision