Effects of KiSS-1 on Human Pancreatic Carcinoma Capan-2 Cell Proliferation and Invasion

Objective To investigate the effects of KiSS-1 on the proliferation and invasion abilities of human pancreatic carcinoma Capan-2 cells.Metods Transient transfection with Lipofectamine was used to up-regulate the expression of KiSS-1 in Capan-2 cells.Tested the expression of KiSS-1 mRNA and Kisspeptin-metastin by real-time quantitative PCR and Western blot.MTT assay was used to detect the influence of KiSS-1 on Capan-2 cell proliferation.Transwell assay with Matrigel coated was used to evaluate the in vitro invasion ability.Results The KiSS-1 mRNA expression of Capan-2 cells was significantly up-regulated 36 hours after transfection.KiSS-1 mRNA and metastin reached the peak 48 hours after transfection.Compared with empty plasmid transfected group and blank control group.invasion ability and invasion index of Capan-2 cell was significantly decrease 48 hours after KiSS-1 transfection(P 0.01),meanwhile,the inhibition rates of Capan-2 cell invasiveness was 56.5%.There were no significant difference of proliferation after transfection compared with empty plasmid group and blank control group(P 0.05).Conclusion KiSS-1 is a metastasis suppressor gene of pancreatic cancer,which can be used as a novel target for gene therapy.