Molecular diversity analysis using Simple sequence repeats (SSRs) in ‘A’ and ‘R’ lines of Ogura CMS system in Indian mustard

Fifty-eight SSR primers were used for molecular diversity analysis among 45 ‘A’ and ‘R’ lines (5 A lines and 40 restorers) of Indian mustard. Out of 58 primers 33 primers were found to be polymorphic. A total of 95 alleles were detected across all 45 genotypes. The PCR amplified products ranged from 70 bp to 350 bp in size. The number of alleles per locus varied from 2 to 5 with an average of 2.89. The PIC value varied from 0.206 to 0.749 with an average of 0.519. The highest PIC value was obtained for BG 132 (0.749) followed by BG 114 (0.744). The UPGMA cluster analysis suggested demarcation of 45 genotypes into six distinct groups in dendrogram wherein three groups had single genotype (restorer line) each. Cluster VI consisted of all five Ogura CMS lines only. In the remaining clusters, restorer lines were distributed. Based on similarity coefficient, cross combinations viz., RH 0749-OA & IOR-17, RH 0749-OA & IOR-20 and RH 119-OA & OR-17 were identified as diverse and might be suitable for using parent in heterosis exploitation in Indian mustard.