The biochemical mechanism of action of proquazone with special reference to the metabolism of connective tissue (biochemical studies for the determination of antiinflammatory effectiveness)

A method is proposed to determine the specific radioactivity of proteokeratan sulfate and proteochondroitin sulfate after incorporation of 35SO4 and 3H-glucosamine. Proquazone inhibits the incorporation of 35SO4 in proteochondroitin sulfate and also in proteokeratan sulfate. Also the incorporation of 3H-glucosamine in proteokeratan sulfate and proteochondroitin sulfate is inhibited. A mechanism is proposed in which Proquazon inhibits the core protein synthesis of proteoglycans and the secondary biosynthesis of the glycosaminoglycan chains. In our opinion the action of an anti-inflammatory drug is based on the inhibition of the proteoglycan synthesis in the anabolic phase of the inflammation.