The altered gene profile of human osteosarcoma cell after knock-down of double functional gene apurinic/apyrimidinic endonuclease

Objective To explore the molecular mechanism of inhibited malignant phenotype by knock-down of apurinic/apyrimidinic endonuclease(APE1) in human osteosarcoma cell HOS,as well as the possible interacting molecules among APE1.Methods Knock-down of APE1 by synthesized APE1 siRNA in HOS cell was identified first by Western blotting and AP endonuclease assay,then the gene profile was determined with GEArray~(TM) series Human CancerPathwayFinder gene array.Results The specific knock-down of APE1 was found in HOS cell transfected with APE1 siRNA,and its inhibited rate was 91.5%.Six biological pathways of the gene array were involved in HOS cell after knock-down of APE1,but the cell cycle pathway was less influenced.Forty-two out of 96 genes were altered(43.8%),in which only CDK4,FGFR2,KAI1 and NCAM were increased,38 others were decreased.Conclusion The knock-down of APE1 was detected to be involved in all the pathways including cell cycle and DNA repair,apoptosis,signal transduction,adhesion,angiogenesis,invasion and metastasis.These findings are significant to further elucidate APE1 role in tumor genesis and to provide experimental evidence for gene therapy targeting APE1 gene in the future.