Evidence for auto/paracrine actions of vitamin D in bone: 1α-hydroxylase expression and activity in human bone cells

Vitamin D is an important regulator of mineral homeostasis and bone metabolism. 1α-Hydroxylation of 25-(OH)D3 to form the bioactive vitamin D hormone, 1α,25-(OH)2D3, is classically considered to take place in the kidney. However, 1α-hydroxylase has been reported at extrarenal sites. Whether bone is a 1α,25-(OH)2D3 synthesizing tissue is not univocal. The aim of this study was to investigate an autocrine/paracrine function for 1α,25-(OH)2D3 in bone. We show that 1α-hydroxlase is expressed in human osteoblasts, as well as the vitamin D binding protein receptors megalin and cubilin. Functional analyses demonstrate that after incubation with the 1α-hydoxylase substrate 25-(OH)D3, the osteoblasts can produce sufficient 1α,25-(OH)2D3 to modulate osteoblast activity, resulting in induced alkaline phosphatase (ALP) activity, osteocalcin (OC) and CYP24 mRNA expression, and mineralization. The classical renal regulators of 1α-hydroxylase, parathyroid hormone, and ambient calcium do not regulate 1α-hydroxylase in os...